molecular stuff
I spent almost a year doing molecular biology. Cloning and such. I am doing that again but somehow, in a different lab, things are different. I feel like i have to start from scratch and thats frustrating cos its been such a long while since i did PCR or ligations, and I didn't make copies of my previous protocols. So I am back to the start, figuring out every little thing and its frustrating.12 is the number for this week 2. After 2 attempts to purify a fragment, I have only 12ng/ul after the ordeal. First using Qiagen's gel extraction, today with Zymo's DNA purification (which is really simple and quick).
Now to my dismay, I noticed that the 'unique' site that I wanted to use is not really unique. In fact, it cuts right in my sequence of interest.
And Vector NTI really ought to have some simple intro/tutorial. I mean, its crazy that no matter how i try to subclone 2 fragments together, their ends never seem to be compatible. It worked once... but not today. I think its a difficult software to use with all its sophistication. Clone - i used that, was something i could figure out after tinkering with it. So was this alignment programme.
I get obsessive. When i want to get something done, sometimes i forget to stop. And i take so long cos sometimes i go off tangent. -- this explains why i am still in office 2 hours after the official work hours.
I need to get a life!
Labels: food
posted by eline @ 7:50 PM
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