Wednesday, March 15, 2006

nothing much really

I just had such awful bread for breakfast!
It was some cheese-sugar bun you commonly see in bakeries. I bought it at one near the market and it totally sucks. Granted that I bought it yesterday, but still, I have never tasted such awful overnight bread before! Urgh! I think I can bake better (ok, I don’t make bread…)

Work:
Did restriction analysis yesterday because previous day’s digestion didn’t exactly give the desired results. I was 4 preps of plasmids with different restriction enzymes. Some time after lunch, i took a look at the gel and it was petrifying! I mean, the bands were awfully faint. It was really barely visible. The DNA ladder didn’t look too either. So I repeated the expt, this time with double the plasmid DNA, and by 5pm, phew, finally got what I wanted. Though still, I have a slight problem with the size. Anyway, guess, its safer to do some sequencing at the 2 ends to make sure everything is ok… eww

Molecular work… I thought I left it 2 years ago..