And somehow, my preferences have evolved over the years. Now, all i want to do is watch simple movies. movies with predictable and feel good endings. Often referred to as chick flicks, romantic comedies. Just today, out of the blue, I thought of Bruce Springsteen's Secret Garden. Part of the soundtrack in Jerry Maguire. I realized that I really like Jerry Maguire. First, i had a largest crush on Tom Cruise when i was in high school
"I love him for the man he wants to be, and i love him for the man that he almost is."
Second, Renee Zellweger gave a superb performance. I love her.
"Come on Jerry, you know this isn't easy for me"
"i am not getting you get rid of me... you, complete me"
"you had me at Hello"
I don't even know why i am going on about movies. Its 7.41pm on a thursday evening. I have an anesthetized maggot on the microscope. 500ms exposure, 488 laser, capture 500 time points at maximum speed. Sensitivity set to 200. Laser power variable. Genotype: dicer;; D42>ANF::GFP/+ vs dicer;; D42>ANF::GFP/UAS,Luciferase RNAi Tripp.
Question: Does the ratio of Gal4 to UAS matter in imaging ANF transport?
Why am i asking this question?
Argh, i dont even want to talk about it now.
So, on to the other more exciting project. My holy grail that could tie up Autophagy, longevity, disease, mitochondria defects etc etc etc...
posted by eline @ 10:49 AM
Comments: 0
